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It is estimated that approximately 11 million people may be infected with this parasite Kurdova-Mintcheva et al. Outbreaks of trichinellosis in humans have been documented in different areas of the world Kurdova-Mintcheva et al. However, control and prevention of this parasite has been difficult due to interconexions among epidemiological cycles and the lack of effective parasite surveillance system in many countries Gottstein et al.
The development of a simple, rapid and accurate diagnostic method for the detection of Trichinella infection in domestic animals is important for effective control and surveillance of this disease. Currently, the clinical diagnosis of trichinellosis is very difficult because most Trichinella infections are asymptomatic or with non-specific clinical manifestations Gottstein et al.
Microscopic examination and serological assays are used to diagnosis of Trichinella infection in domestic or wild boars Gottstein et al. Microscopic examinations are routinely used for the detection of Trichinella larvae in muscle tissues at slaughtering. However, the microscopic examination is labor-intensive, low sensitive, time-consuming, and also requires the use of microscope and a trained personnel Gottstein et al. Serological assays have been useful for epidemiological studies and large-scale disease surveillance, but these immunologic diagnostic methods cannot replace the direct detection methods used for meat inspection due to the potential cross-reactivity with other parasites Gottstein et al.
Although PCR-based assays are highly sensitive and can detect low parasite burdens, they require expensive instruments and a trained technician, making the use of PCR based-methods difficult in resource-limited settings Gottstein et al.
Therefore, a rapid, sensitive, specific, and field-applicable diagnostic method is clearly desired to improve the effectiveness of Trichinella control and surveillance programs. The recombinase polymerase amplification RPA , an isothermal DNA amplification technology, has been developed for the diagnosis of several pathogens James and Macdonald, ; Daher et al.
This RPA technique does not require the use of thermal cycling apparatus to denature DNA template, but instead utilizes recombinase-primers to scan for homologous sequences in a DNA template and facilitates DNA strand exchange at cognate sites James and Macdonald, ; Daher et al.
Additionally, RPA DNA amplified products can be detected by simple lateral flow LF strips and the results can be easily read without any specialized equipment, which provides convenient diagnostic assay for the detection of Trichinella infection in resource-limited settings James and Macdonald, ; Daher et al.
The sensitivity and specificity of this assay were investigated in comparison with a conventional PCR assay. In addition, the effectiveness of LF-RPA was evaluated in the presence of PCR inhibitors of the muscle and in samples obtained from experimentally infected mice.
Primers and a probe specific to the rrnS gene of Trichinella were designed according to the instruction manual of TwistDX. Seperti cerita tentang Lupus yang rela latihan Paskibra untuk bisa mendekati annk kelas satu yang manis, terus cerita tentang Lulu yang punya baju putih favorit tapi akhirnya harus rela kehilangan baju tersebut, atau juga cerita Lupus yang ditinggal teman-temannya yang mau liburan ke Bandung.
Wedding Women's Interest. International Jawa. Kalimantan National. About Hilman Hariwijaya. Hilman Hariwijaya. Hilman yang turunan Jasun alias Jawa-Sunda ini punya papa tentara berpangkat kolonel.
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